Sequencing Runs¶
When VariantGrid has access to a network drive (eg a diagnostic lab intranet) it can scan disks for sequencing runs to collect QC metrics, gene coverage and automatically load VCFs.
Sequencing Samples over time
Automatically loaded sequencing runs + VCFs
A Sequencing Run
We collect Sequencing QC metrics and display them with interactive graphs. Collecting data over time allows us to see how this run compares to other runs over time (or vs gold standard runs).
Gold Standard Runs¶
The administrator can mark a sequencing run as “Gold Standard” - which means it has passed validation / is of sufficient quality to be used as a benchmark for other runs.
Gold standard runs have an icon () on the sequencing run grid.
Gold runs for an enrichment kit are used:
In boxplots on QC metrics pages for a sequencing run or other sample QC graphs.
To calculate average gene coverage on the GeneGrid page.
Finding sequencing data¶
Sequencing Runs are found by searching for the file ‘RTAComplete.txt’ on the server disks. You can ignore flow cells by putting a file “.variantgrid_skip_flowcell” in the directory.